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KMID : 0380020060210040286
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 4 p.286 ~ p.291
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Fermentation Kinetics for Production of Carotenoids by β-ionone Resistant Mutant of Xanthophyllomyces dendrorhous
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Park Ki-Moon
Kim Young-Jun
Song Min-Woo
Kang Seog-Jin
Lee Jae-Heung
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Abstract
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Various β-ionone resistant mutants were isolated from the wild-type red yeast Xanthophyllomyces dendrorhous KCTC 7704. Although the growth of X. dendrorhous KCTC 7704 was strongly inhibited at 0.025 mM β-ionone, one of the β-ionone resistant mutants isolated at 0.1 mM β-ionone by NTG mutagenesis showed rather 70% of relative survival at 0.15 mM β-ionone. Fermentation kinetics study with the mutant was carried out at 20℃ for 4 days in 300-mL baffled flasks. The mutant yielded up to 2.3-fold higher carotenoids content (viz. 1.2 μg of total carotenoids per mg of dry cells) compared with the wild-type strain. The production of metabolites such as organic acids could be neglected. Studies on the kinetics with various carbon substrates revealed both an increase in final dry cell mass and a higher total carotenoids content in cell mass with glucose when compared to fructose or sucrose. As a further part of study, the effect of pH on the fermentation kinetics was investigated in glucose-limited chemostat at a dilution rate of 0.04 h-1. When compared to steady-state kinetic parameters obtained at pH 4.0, a significant reduction in cell concentration at pH 3.0 and a lower carotenoids content at pH 5.2 were evident.
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KEYWORD
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Astaxanthin, chemostat, fermentation kinetics, β-ionone, total carotenoids
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